Aflatoxin detoxification by manganese peroxidase purified from Pleurotus ostreatus
نویسندگان
چکیده
منابع مشابه
Aflatoxin detoxification by manganese peroxidase purified from Pleurotus ostreatus
Manganese peroxidase (MnP) was produced from white rot edible mushroom Pleurotus ostreatus on the culture filtrate. The enzyme was purified to homogeneity using (NH4)2SO4 precipitation, DEAE-Sepharose and Sephadex G-100 column chromatography. The final enzyme activity achieved 81 U mL(-1), specific activity 78 U mg(-1) with purification fold of 130 and recovery 1.2% of the crude enzyme. SDS-PAG...
متن کاملDirect oxidation of polymeric substrates by multifunctional manganese peroxidase isoenzyme from Pleurotus ostreatus without redox mediators.
VPs (versatile peroxidases) sharing the functions of LiP (lignin peroxidase) and MnP (manganese peroxidase) have been described in basidiomycetous fungi Pleurotus and Bjerkandera. Despite the importance of this enzyme in polymer degradation, its reactivity with polymeric substrates remains poorly understood. In the present study, we first report that, unlike LiP, VP from Pleurotus ostreatus dir...
متن کاملBio-detoxification of Jatropha curcas seed cake by Pleurotus ostreatus
The detoxification of Jatropha curcas seed cake is of major interest for the biodiesel industry to add economic value to this residue and also to reduce the environmental damage caused by its inappropriate disposal. In this context, the treatment of this residue with white rot fungus, Pleurotus ostreatus, can be a viable alternative because it produces enzymes capable of degrading different lig...
متن کاملRedundancy among manganese peroxidases in Pleurotus ostreatus.
Manganese peroxidases (MnPs) are key players in the ligninolytic system of white rot fungi. In Pleurotus ostreatus (the oyster mushroom) these enzymes are encoded by a gene family comprising nine members, mnp1 to -9 (mnp genes). Mn(2+) amendment to P. ostreatus cultures results in enhanced degradation of recalcitrant compounds (such as the azo dye orange II) and lignin. In Mn(2+)-amended glucos...
متن کاملPleurotus ostreatus manganese‐dependent peroxidase silencing impairs decolourization of Orange II
Decolourization of azo dyes by Pleurotus ostreatus, a white-rot fungus capable of lignin depolymerization and mineralization, is related to the ligninolytic activity of enzymes produced by this fungus. The capacity of P. ostreatus to decolourize the azo dye Orange II (OII) was dependent and positively co-linear to Mn(2+) concentration in the medium, and thus attributed to Mn(2+)-dependent perox...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2014
ISSN: 1517-8382
DOI: 10.1590/s1517-83822014005000026